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本研究利用果蝇模型，发现srd基因突变会导致精子再生障碍。Srd蛋白在其精巢中异性表达，其缺失引起精巢内活性氧（ROS）含量升高，引起再生精子活力下降，聚集于精巢终末端无法进入储精囊。实验使用抗氧化剂N-乙酰-L-半胱氨酸（NAC）清除ROS，可有效改善srd基因突变体精子再生障碍。该研究结果揭示了Srd蛋白通过调控ROS稳态来维持精子再生的机制，为理解精子再生障碍及男性不育诊疗提供了潜在靶点。
Abstract：Using a Drosophila model, this study reveals that mutation of the srd gene leads to sperm regeneration disorder. The Srd protein is specifically expressed in the testis, its loss elevates reactive oxygen species(ROS)levels in the testis, impairing the motility of regenerating sperms and causing them to accumulate at the testicular distal end, thereby failing to migrate into the seminal vesicle. Administration of the antioxidant N-acetyl-L-cysteine(NAC) to scavenge ROS effectively ameliorates the sperm regeneration disorder in srd mutants. This study elucidates the mechanism by which Srd maintains sperm regeneration via regulating ROS homeostasis, providing a potential molecular target for understanding sperm regeneration disorder and the diagnosis and therapeutic intervention of male infertility.
[1]ANDRABI S W, ARA A, SAHARAN A, et al. Sperm DNA fragmentation:causes, evaluation and management in male infertility[J]. JBRA Assisted Reproduction, 2024,28(2):306-319.
[2]EVENSON D P, DJIRA G, KASPERSON K, et al.Relationships between the age of 25,445 men attending infertility clinics and sperm chromatin structure assay(SCSA®)defined sperm DNA and chromatin integrity[J].Fertility and Sterility, 2020, 114(2):311-320.
[3]VEDELEK V, JANKOVICS F, ZáDORI J, et al.Mitochondrial differentiation during spermatogenesis:lessons from Drosophila melanogaster[J]. International Journal of Molecular Sciences, 2024, 25(7)ijms25073980.
[4]WALKE G, GAURKAR S S, PRASAD R, et al. The impact of oxidative stress on male reproductive function:Exploring the role of antioxidant supplementation[J].Cureus, 2023, 15(7):e42583.
[5]毋肇琪,曹进国.青牛胆对阿尔茨海默病果蝇模型的抗炎与自噬保护作用研究[J].井冈山大学学报(自然科学版), 2025, 46(5):44-55.
[6]WANG X, ZHOU P, ZHANG Z, et al. A Drosophila model of gestational antimony exposure uncovers growth and developmental disorders caused by disrupting oxidative stress homeostasis[J]. Free Radical Biology&Medicine, 2023, 208:418-429.
[7]FOUTOUHI A, MEYERS S. Comparative oxidative metabolism in mammalian sperm[J]. Animal Reproduction Science, 2022, 247:107095.
[8]KALYANARAMAN B. NAC, NAC, Knockin'on heaven's door:interpreting the mechanism of action of n-acetylcysteine in tumor and immune cells[J]. Redox Biology, 2022, 57:102497.
[9]KOTHALAWALA S D, GüNTHER S, SCHUPPE H C, et al. Identification of differentially expressed genes in human testis biopsies with defective spermatogenesis[J].Reproductive Medicine and Biology,2024, 23(1):e12616.
[10]KAUSHAL N, BANSAL M P. Dietary selenium variation-induced oxidative stress modulates CDC2/cyclin B1 expression and apoptosis of germ cells in mice testis[J]. The Journal of Nutritional Biochemistry, 2007,18(8):553-564.
[11]ZHUANG X, SEMENOVA E, MARIC D, et al.Dephosphorylation of barrier-to-autointegration factor by protein phosphatase 4 and its role in cell mitosis[J]. The Journal of Biological Chemistry, 2014, 289(2):1119-1127.
[12]LIU M, LIU S, SONG C, et al. Pre-meiotic deletion of PEX5 causes spermatogenesis failure and infertility in mice[J]. Cell Proliferation, 2023, 56(3):e13365.
[13]WANG C, MA Z, SCOTT M P, et al. The cholesterol trafficking protein NPC1 is required for Drosophila spermatogenesis[J]. Developmental Biology, 2011,351(1):146-155.
[14]MIAO L, ST CLAIR D K. Regulation of superoxide dismutase genes:implications in disease[J]. Free Radical Biology&Medicine, 2009, 47(4):344-356.
[15]DRöGE W. Free radicals in the physiological control of cell function[J]. Physiological Reviews, 2002, 82(1):47-95.
[16]叶万里,汪海.血清PTEN、ApoB/ApoA-Ⅰ及精浆活性氧水平与不育男性患者精液质量的相关性[J].中国性科学, 2025, 34(12):18-23.
[17]马涛,刘冬立.硝酸钇暴露诱发小鼠睾丸氧化应激损伤精子质量的研究[J].包头医学院学报,2025,41(1):32-37.
[18]ZHANG X, PENG J, WU M, et al. Broad phosphorylation mediated by testis-specific serine/threonine kinases contributes to spermiogenesis and male fertility[J]. Nature Communications, 2023, 14(1):2629.
基本信息:
中图分类号:Q344
引用信息:
[1]原移钦,胡煜辉,曹进国.Srd调控果蝇精子再生机制研究[J].井冈山大学学报(自然科学版),2026,47(03):26-32.
基金信息:
国家自然科学基金项目（32160177）; 江西省教育厅科技计划项目（GJJ2201622）
2026-05-10
2026-05-10